Self-paced HPLC introduction. We provide you with interactive HPLC introduction documents for your self-study. Our HPLC familiarization documents cover basic introduction and operation of our HPLC instruments, ensuring you have a positive start-up experience. Incomplete HPLC familiarization can lead to improper use of the instrument, so make
Septa are made from different materials. See below for definitions and uses of different types of septa materials. Rubber septa. Used primarily for routine analysis in gas chromatography. Offers moderate ability to reseal and good chemical inertness. Not recommended for multiple injections or holding samples for further analysis.
Aug 28, 2017 · Various grades and types of septa are available including septa specifically designed to minimise leachates into the sample solution. Figure 5: Comparison of the effect of wearing different laboratory glove types when preparing aqueous mobile phase for HPLC. Comparison of baseline profiles (254 nm) obtained following enrichment of 60 mL aqueous
caps with slit septa. • Never shake the vial. If you do there is going to be sample on the underside of the septum which gives carry over. • Adjust the needle height according to the vial dimensions. • Rubber septas get dry and can block the injector.
Jul 31, 2020 · Needle Gauge: A bigger needle gauge can need more force to get through non-pre-slit septa, which could cause damage to the septa or sample contamination. A pre-slit septum might be the best choice in this situation. Injection Frequency: A pre-slit septa may be more appropriate for applications requiring repeated injections since it can lessen
Mar 31, 2022 · The behavior of the volatile analytes differs significantly between headspace and direct injection. Most of the sample material reaches the intake system when injecting a sample into a GC inlet. 2. Which vial should a person use for HPLC? PTFE/Silicone septa (T/S) are the most suitable for HPLC applications.
most HPLC autosamplers. Polypropylene septa are not resealable and are intended for single injection use with aqueous based sample mixtures. Polypropylene septa offer better solvent compatibility compared to polyethylene, but piercing force is slightly higher. Viton (V1) septa Viton septa are used in situations where a resealable septum is
Septa are made from different materials. See below for definitions and uses of different types of septa materials. Rubber septa. Used primarily for routine analysis in gas chromatography. Offers moderate ability to reseal and good chemical inertness. Not recommended for multiple injections or holding samples for further analysis.
• Single-layered septa are acceptable if they do not contain plastics or adhesives that can contaminate the sample manager. (PTFE is recommended.) c. Use Waters-brand wellplates. Be aware that other brands may leach plasticizers (e.g., diisooctylphthalates). d. Foil-lined plate covers are acceptable as long as the aluminum does not touch the
allows for smoother penetration of vial and injector septa. SPME fiber advantages: – Original proven design – Solvent-free – Easy to automate – Nondestructive to samples – Fibers are reusable – Compatible with GC or HPLC instrumentation All Aijiren SPME fibers have a standard length of 10 mm and a 23-gauge needle.
Septa are made from different materials. See below for definitions and uses of different types of septa materials. Rubber septa. Used primarily for routine analysis in gas chromatography. Offers moderate ability to reseal and good chemical inertness. Not recommended for multiple injections or holding samples for further analysis.
When selecting a vial, cap and septum to contain analytical solutions, there are several factors to take into account. In most analyses, the cleanliness of the vial and the septum are most important. 1,2 With analyses where multiple injections of the same analyte (s) are performed over time, the ability of the septum to reseal is a consideration.
• Can change to sample from different heights in the vial - A setpoint of -2 mm will sample 1.6 mm from the vial bottom - Range is -2 mm to 30 mm • Example uses: - Samples with sediment (although properly filtering the sample is ideal) - Sampling from higher in the sample vial in liquid-liquid extractions - Small volume sampling
vial-cap combination provides the best seal for long term sample storage, as well as high volatility solvents. 12 x 32 mm, 11 mm Crimp-Top Vials and Closures Crimp-Top Vials, 2.0 mL • Cleaner vials eliminate ghost peaks and contaminants • ®Used with most autosamplers, including Aijiren ®, Thermo Scientific, and Waters
Aug 22, 2023 · Optimal Septa Selection. Precision in septa selection is paramount: Material Matchup: Choose from silicone, rubber, or inert PTFE. PTFE septa are excellent for samples sensitive to contamination. Puncture Perfection: Septa must be puncture-friendly to injection needles, preventing coring or excessive wear.
